Division of Life Science
 
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Tuan Anh NGUYEN

PhD Korea Advanced Institute of Science and Technology (KAIST)
Assistant Professor

Email: tuananh@ust.hk

 

Research Interests

RNA Biochematics Lab

MicroRNAs (miRNAs) are small RNAs of ~22 nucleotides in length which do not encode any proteins but play critical roles in the posttranscriptional regulation of gene expression. In humans, many miRNAs involve in various cellular processes and associate with numerous human diseases, including cancers and neurodegenerative diseases. Each miRNA sequence is embedded in its longer RNA precursor transcribed from the genome, namely pri-miRNA, and has its expression profiles which vary depending on cellular contexts. Pri-miRNAs express markedly diverse features in their primary sequences and secondary structures, many of which are critical to expressing miRNAs. Our lab main focus is to comprehensively understand how the modulated and structured features of pri-miRNAs are translated into their distinctive miRNA expression via multiple molecular mechanisms involving RNA-interacting proteins and enzymes as well as their auxiliary factors, including small chemical molecules. To elucidate these mechanisms, we employ multidisciplinary approaches, including biochemistry and bioinformatics techniques.

Representative Publications


  1. Kim K*, Nguyen TD*, Li SH, Nguyen TA. (2018) SRSF3 recruits DROSHA to the basal junction of primary microRNAs. RNA in press.*Co-first authors.
     
  2. Nguyen TA*, Park J*, Dang TL, Choi YG, Kim VN. (2018) Microprocessor depends on hemin to recognize the apical loop of primary microRNA. Nucleic Acids Res in press. *Co-first authors, co-corresponding authors.
     
  3. Kwon SC*, Nguyen TA*, Choi YG*, Jo MH, Hohng S, Kim VN and Woo JS. (2016) Structure of human DROSHA. Cell 164, 81–90. *Co-first authors. Highlighted in Cell Research (2016) 26:511–512; Video abstract by Cell journal.
     
  4. Nguyen TA, Jo MH, Choi YG, Park J, Kwon SC, Hohng S, Kim VN and Woo JS. (2015) Functional anatomy of the human Microprocessor. Cell 161:1374–1387.
    Top 10 Science News of the Year by the Korean Federation of Science and Technology Societies (KOFST); Top 5 Basic Academy by Biological Research Information Center (BRIC); F1000Prime.
     
  5. Thu HP*, Nguyen TA*, Munashingha P, Kwon BK, Dao Van Q and Seo YS. (2015) A physiological significance of the functional interaction between Mus81 and Rad27 in homologous recombination repair. Nucleic Acids Res 43:1684–1699. *Co-first authors.
     
  6. Lee M, Choi Y, Kim K, Jin H, Lim J, Nguyen TA, Yang J, Jeong M, Giraldez AJ, Yang H, Patel DJ and Kim VN. (2014) Adenylation of maternally inherited microRNAs by Wispy. Molecular Cell 56:696–707.
     
  7. Kang JH, Lee CH, Munashingha P, Nguyen TA and Seo YS. (2012) Biochemical studies of the Saccharomyces cerevisiae Mph1 helicase on junction-containing DNA structures. Nucleic Acids Res 40:2089–2106.
     
  8. Shin YK, Amangyeld T, Nguyen TA, Munashingha PR, Seo YS. (2012) Human MUS81 complexes stimulate flap endonuclease 1. FEBS J 279:2412–2430.
     
  9. Munashingha PR, Lee CH, Kang H, Shin YK, Nguyen TA, Seo YS. (2012) The transauto-stimulatory activity of Rad27 suppresses dna2 defects in Okazaki fragment processing. J Biol Chem 16:8675–8687. 2012.
     
  10. Nguyen TA, Tak YS, Lee CH, Kang YH, Cho IT and Seo YS. (2011) Analysis of subunit assembly and function of the Saccharomyces cerevisiae RNase H2 complex. FEBS J 287:4927–4942.
     
  11. Nguyen TA, Lee CH and Seo YS. (2011) Lagging Strand Synthesis and Genomic Stability, DNA Repair-On the Pathways to Fixing DNA Damage and Errors, Francesca Storici (Ed.). DNA repair-On the Pathways to Fixing DNA Damage and Errors, InTech, Croatia, 1:1–24.
     
  12. Kang MJ, Lee CH, Kang YH, Cho IT, Nguyen TA and Seo YS. (2010) Genetic and functional interactions between Mus81/Mms4 and Rad27. Nucleic Acids Res 38:7611–7625.
     
  13. Lee CH, Shin YK, Phung TT, Bae JS, Kang YH, Nguyen TA, Kim JH, Kim DH, Kang MJ, Bae SH and Seo YS. (2010) Involvement of Vts1, a structure-specific RNA-binding protein, in Okazaki fragment processing in yeast. Nucleic Acids Res 38:1583–1595.
     
  14. Cho IT, Kim DH, Kang YH, Lee CH, Amangyelid T, Nguyen TA, Hurwitz J and Seo YS. (2009) Human replication factor C stimulates flap endonuclease 1. J Biol Chem 284:10387–10399.