Division of Life Science
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Guang ZHU

PhD Maryland

Email: gzhu@ust.hk

Homepage of Zhu's research lab

Research Interests
Structure-function study of proteins involved in the initiation of DNA replication, epigenetic control of gene expression and cancer. NMR study of tRNA and DNA/RNA G-quadruplex. Development of NMR methods for structure-function studies of biomolecules.


Refereed Publications

1.      Guang Zhu and Ad Bax (1990) Improved Linear Prediction for Truncated Signals of Known Phase. J. Magn. Reson. 90,405-410,   (times cited 224)

2.      Lewis E. Kay, Mitsuhiko Ikura, Guang Zhu and Ad Bax (1991) Four-Dimensional Heteronuclear Triple Resonance NMR of Isotopically Enriched Proteins for Sequential assignment of Backbone atoms. J. Magn. Reson.91,422-428 (times cited 42)

3.      Ad Bax, Mitsuhiko Ikura, Lewis E. Kay and Guang Zhu (1991) Removal of F1 Baseline Distortion and Optimization of Folding in Multi-Dimensional NMR Spectra. J. Magn. Reson. 91,174-178 (times cited 142)

4.      Lewis E. Kay, Mitsuhiko Ikura, Guang Zhu and Ad Bax (1991) 3-Dimensional and 4-Dimensional Heteronuclear Triple Resonance NMR Spectroscopy of Proteins in Solution. Abstracts of Paper of the American Chemical society. 201, APR. 74,

5.      Guang Zhu and Ad Bax (1992) Two-dimensional Linear Prediction for Signals Truncated in Both Dimensions. J. Magn. Reson. 98, 192-199 (times cited 60)

6.      M.L. Rustgi, L.N. Pandey, J.W.Wilson, S.A.T. Long and Guang Zhu (1988) Distribution of Energy in Polymers Due to Incident Electrons and Protons. Radiation Effec. and Defec. in Solids 105 3-4 303

7.      Guang Zhu and Ad Bax (1992) Improved Linear Prediction of Truncated Damped Sinusoids Using modified Backward-Forward Linear Prediction. J.Magn.Reson. 100, 202-207 (times cited 85)

8.      M. Ikura, G.M. Clore, A.M. Gronenborn, Guang Zhu, C.B. Klee, and A. Bax, (1992) Solution Structure of a Calmodulin-Target Peptide Complex by Multidimensional NMR. Science, 256, 632-638 (times cited 1001)

9.      Guang Zhu and Ad Bax (1993) Quantitative Measurement of Long-range 1H-13C J Couplings from Proton Detected HMBC Spectra. J. Magn. Reson. Series A 104, 353-357 (times cited 46)

10.  Guang Zhu, Denis Torchia and Ad Bax (1993) Discrete Fourier Transformation of NMR Signals. The Relationship between Sampling Delay Time and Spectral Baseline. J. Magn.Reson. Series A 105, 219-222 (times cited 25)

11.  Ad Bax, Geerten W. Vuister, Stephan Grzesiek, Frank Delaglio, Andy C. Wang, Rolf Tschudin, and Guang Zhu (1994) Measurement of homo- and heteronuclear J couplings from quantitative J correlation. Methods in Enzymology Vol. 239,79-105 (times cited 330)

12.  Guang Zhu, David Live and Ad Bax (1994) Analysis of Sugar Puckers and Glycosidic Torsion Angles in a DNA G-Tetrad Structure by Heteronuclear Three-Bond J Couplings. J. Am. Chem Soc.116, 8370-71 (times cited 47)

13.  Guang Zhu, Alistair Renwick, and Ad Bax (1994) Measurement of two- and three-bond 1H-13C J couplings from quantitative heteronuclear J correlation for molecules with overlapping 1H resonances, Using T1 Noise Reduction. J. Magn. Reson Series A 110, 257-261 (times cited 33)

14.  Guang Zhu, David Live and Ad Bax (1994) Stable Isotope Applications in Biomolecular Structure and Mechanisms, (edited by J. Trewhella, T.A. Cross and C. J. Unkefer), Los Alamos, New Mexico, pp345,

15.  Frank Delaglio[1], Stephen Greziesk, Geerten Vuister, Guang Zhu, Johon Pfeifer and Ad Bax*1. (1995) nmrPipe: A multidimensional spectral processing system based on Unix pipes. J. Biomol. NMR, 6 277-293 (times cited 5839)

16.  Guang Zhu* (1996) Application of a Three-Dimensional Maximum Entropy Method to Processing Sections of Three-Dimensional NMR Spectra. J.Magn.Reson Series B 113, 248-251 (times cited 3)

17.  Guang Zhu*, and Yinbo Hua1 (1997) Quantitative NMR spectral analysis by an interative maximum likelihood method. Chem. Phys. Lett  264, 424-429 (times cited 10)

18.  Guang Zhu*, David Smith2 and Yinbo Hua1 (1997) Post-Acquisition Solvent Suppression By Singular Value Decomposition. J. Magn. Reson 124, 286-290 (times cited 24)

19.  Wingyiu Choy4, B.C. Sanctuary1 and Guang Zhu* (1997) Using Neural Network Predicted Secondary Structure and Information in Automatic Protein NMR Assignment. J. Chem. Info. Comp. Sci. 37, 1086-1094 (times cited 16)

20.  Guang Zhu*, Wingyiu Choy4, B.C. Sanctuary1, and Guoqiang Song4 (1998) Suppression of Diagonal peaks by Singular Value Decomposition. J. Magn. Reson. 132 176-178 ( times cited 7)

21.  Guang Zhu*, Wingyiu Choy4 and B.C. Sanctuary1 (1998) Spectral parameter estimation by an iterative maximum likelihood method. J. Magn. Reson. 135, 37-43 (times cited 7)

22.  Guang Zhu*, Xiangming Kong4, and Konghong Sze5 (1998) Gradient and Sensitivity Enhanced HMQC experiment. J. Magn. Reson. 135, 232-235 (times cited 9)

23.  Guang Zhu*, Xiangming KONG4, Xianzhong YAN3, and Konghong SZE5 (1998) Sensitivity Enhancement of Transverse Relaxation-optimized Spectroscopy. Angew. Chem. Int. Ed. Engl. 37, 2859-2861 (it was selected as a “hot” paper) (times cited 18)

24.  Guang Zhu*, Xiangming Kong4 and Konghong Sze5 (1999) Gradient and sensitivity enhancement of 2D TROSY with water flip-back, 3D NOESY-TROSY and TOCSY-TROSY experiments J. Biomol. NMR, 13 (1): 77-81 1999 (Times Cited 54)

25.  Xiangming Kong4, Konghong Sze5, and Guang Zhu* (1999) Gradient and Sensitivity Enhanced Multiple-quantum Coherence and Its Application in Heteronuclear Multi-dimensional NMR Experiments. J. Biomol. NMR, 14, 133-140 (times cited 4)

26.  Konghong Sze5, Xiangming Kong4 and Guang Zhu* (1999) Measuring 3JCH by 3D J-Resolved HMBC Experiment. Tetrahedron lett. 40, 5587-5591 (times cited 5)

27.  Guang Zhu*, Youlin Xia2, Konghong Sze5 and Xianzhong Yan3 (1999) 2D and 3D TROSY Enhanced NOESY of 15N Labeled Proteins. J. Biomol. NMR, 14, 377-381 (times cited 15)

28.  Guang Zhu*, Youlin Xia2, Linda Nicholson1, and Kong Hung Sze5 (2000) Protein dynamics measurements by TROSY-based NMR experiments. J. Magn. Reson. 143,423-426.( times cited 67)

29.  Qingwen Zhang1, Wencai Ye, Xiangzhong Yan3, Guang Zhu, Chuntao Che1 and Shouxun Zhao (2000) Sucrase Inhibitory Sanonins from Pulsatilla Cernua J. Nat. Prod. 63, 276-278 (times cited 3)

30.  Xianzhong Yan3, Hong Xue1, Hongzhi Liu, Jun Hang, J. Tze-Fei Wong1 and Guang Zhu* (2000) NMR Studies of Bacillus subtilis tRNATrp Hyper-expressed in E.coli: Assignment of Imino Proton Signals and Determination of Thermal Stability J. Biol. Chem. 275, 6712-6716 (times cited 9)

31.  Youlin Xia2, Xiangming Kong4, Yu Liu2, David Man3, David Smith2 and Guang Zhu* (2000) Sensitivity Enhancement of HCACO by Using an HMQC Magnetization Transfer Scheme. J. Mang. Reson. 143,407-410 (times cited 8)

32.  Youlin Xia2, Xiangming Kong4, Nancy IP1 and Guang Zhu* (2000) A J-Multiplied HMQC (MJ-HMQC) Experiment for Measuring 3JHNHa Coupling Constants, J. Magn. Reson. 146, 228-231 ( times cited  5)

33.  Xianzhong Yan3, Xiangming Kong4, Youlin Xia2, Konghung Sze5 and Guang Zhu* (2000) Determination of Internucleotide hJHN Couplings by the Modified 2D JNN Correlated-[15N, 1H]-TROSY, J. Magn. Reson. 147, 357-360 (times cited 10)

34.  Youlin Xia2, Kong Hong Sze5 and Guang Zhu* (2000) Transverse Relaxation optimized 3D and 4D 15N/15N separated NOESY 15N labeled of Protein labeled. J.Biomol. NMR 18, 261-268 (times cited 11)

35.  Youlin Xia2, D. Man3, and Guang Zhu* (2001) 3D Haro-NOESY-CH3NH and Caro-NOESY-CH3NH Experiments for Double Labeled Proteins. J. Biomol. NMR 19, 355-360 (times cited 8)

36.  Bingwen LU, Laikwan PEI, Waikin CHAN, Hong ZHANG, Guang Zhu, Jiayang LI and Ning LI1 (2001) The dual effects of ethylene on the negative gravicurvature of arabidopsis inflorescence, an intriguing action model for the plant hormone ethylene, Chinese Science Bulletin 46,1 (times cited 6)

37.  David Man3, Youlin Xia2, Kong H. Sze5, David K. Smith2, Wei He1, Nancy Y. Ip1 and Guang Zhu* (2002) Complete 1H, 15N and 13C assignments of the carboxyl terminal domain of the ciliary ceurotrophic factor receptor (CNTFR), J. Biomol. NMR, 22 95-96 (times cited 2)

38.  Youlin Xia2, Kong Hung SZE5, N. Li1, P.C. Shaw1 and Guang Zhu* (2002) Protein Dynamics Measurements by 3D HNCO Based NMR Experiments, Spectroscopy, an international journal,16, 1-13 (times cited 2)

39.  Donghai Lin2, Kong Hung Sze5, Yanfan Cui4 and Guang Zhu* (2002) Clean sea-HSQC: a method to Map Solvent Exposed Amides in Large Proteins with Gradient-enhanced HSQC, J. Biomol. NMR 23, 317-322 (times cited 15)

40.  Wei He1, Ke Gong, Guang Zhu, David Smith2, Nancy IP1* (2002) Memberane distal cytokine binding domain of LIFR interacts with the soluble CNTFR in Vitro, FEBS Letter 514 214-218 (times cited 9)

41.  Qingguo Gong1, Qing Guo, Guang Zhu, J.F. Wong1 and Hong Xue1 (2002) NMR Analysis of Bovine tRNATrp: Conformation Dependence of Mg2+ Binding Sites, J. Biol.Chem. 277 20694-20701  (times cited 3)

42.  Guang Zhu* (2002) Multidimensional NMR data processing, (Chapter 17, Signal processing for magnetic resonance imaging and spectroscopy. Edited by Hong Yan, PP509-543 Marcel Dekker, Inc, New York)

43.  Qing Guo1, Qingguo Gong1, Ka-Lok Tong, Bente Vestergaard, Annie Costa, Jean Desgres, Mansim Wong3, Henri Grosjean, Guang Zhu, J. Tze-Fei Wong1, and Hong Xue1 (2002) Recognition by Tryptophanyl-tRNA Synthetases of Discriminator Base on tRNATrp from Three Biological Domains. J. Biol. Chem. 277: 14343-14349 (times cited 16)

44.  Ya-Hui Chi, Thallampuranam Krishnaswamy S. Kumar, Dong-Hai Lin2, Guang Zhu, Ing-Ming Chiu and Chin Yu1(2002) Investigation of the Structural Stability of the Human Acidic Fibroblast Growth Factor by Hydrogen-Deuterium Exchange. Biochemistry. 41, 15350-9 (times cited 22)

45.  D. K. Smith1, P. Radivojac, Z. Obradovic1, A. K. Dunker1 and Guang Zhu* (2003) Improved Amino Acid Flexibility ParametersProtein Science 12(5):1060-1072 (times cited 68)

46.  Yanfang Cui4, Jian Wen4, Konghung Sze5, David Man3, Donghai Lin2, Maili Liu1, and Guang Zhu* (2003) Interaction Studies Between Calcium-free Calmodulin and the IQ Motif of Neurogranin Using NMR Spectroscopy. Analytical Biochemistry 15;315(2)175-82. (times cited 16)

47.  Man D.3, Sze K.H.5, Smith D.2, Wei H., Guang Zhu* and Ip N. Y (2003) Solution Structure and Backbone Dynamics of the Carboxy-Terminal Domain of the Cytokine Receptor Homology Region of Ciliary Neurotrophic Factor Alpha Receptor, J. Biol. Chem. 278 (26): 23285-23294  (times cited 16)

48.  Jonathan K.L3. Chan, Luguo Sun, Xiang-jiao Yang, G. Zhu, and Zhenguo Wu1 (2003) Functional characterization of an amino terminal region of HDAC4 that possesses MEF2 binding and transcriptional repressive activity. J. Biol. Chem. 278, 23515-23521 (times cited 31)

49.  Yong-Neng Yao1, Qing-Shuo Zhang1, Xian-Zhong Yan3, Guang Zhu*, and En-Duo Wang1* (2003) Substrate-Induced Conformational Changes in Escherichia coli Arginyl-tRNA Synthetase Observed by 19F NMR spectroscopy, FEBS Letter  547 197-200 (times cited 4)

50.  Radivojac P., Obradovic Z., Smith D.K.1, Guang Zhu, Vucetic S., Brown CJ, Lawson J.D., Dunker A.K.1 (2004) “Protein flexibility and intrinsic disorder” Protein Science 2004 13(1):71-80 (times citied 121).

51.  Guang Zhu*, Youlin Xia, Donghai Lin, and Xiaolian Gao (2004) TROSY-based NMR experiments for the study of macromolecular dynamics and hydrogen bonding, (Chapter 11, Methods in Molecular Biology/Molecular Medicine, Humana Press), 161-184

52.  Youlin Xia1, Guang Zhu and Xiaolian Gao1 (2004) (3,2)D GFT-NMR Experiments for Fast Collection of Protein Data J. Biomol. NMR, 29 (4): 467-476 (times cited 17)

53.  Guang Zhu*, Youlin Xia, Donghai Lin, and Xiaolian Gao (2004) TROSY based correlation and NOE spectroscopy for NMR structural studies of large proteins (Chapter 5, Methods in Molecular Biology/Molecular Medicine, Humana Press) 57-78

54.  H. Chen, Richard K. Haynes, Jürgen Scherkenbeck,  Kong H. Sze5 and Guang Zhu (2004) Elucidation of Solution Conformations of Loloatin C by NMR Spectroscopy Combined with Molecular Simulation, European Journal of  Organic Chemistry 31-37 (times cited 3)

55.  Yong-Neng Yao1, Qing-Shuo Zhang1, Xian-Zhong Yan3, Guang Zhu, and En-do Wang1 (2004) E. Coli tRNAArg (UCU) induces a constrained conformation the crucial W-loop of arginyl-tRNA synthetase. Biochemical and Biophysical Research Communications 313 129-134. (times cited 2)

56.  Kewei Ma, Jonathan K.L.Chan3, Guang Zhu, and Zhenguo Wu1 (2005) Myocyte Enhancer Factor 2 Acetylation by p300 Enhances Its DNA Binding Activity, Transcription Activity, and Myogenic Differentiation. Mol. And Cell. Biol, 3575-3582 (times cited 59)

57.  Yinliang Yin2, Changdong Liu4, Helen Tsai, Bo Zhou3, Sai Ming Ngai1, Guang Zhu* (2005) SET8 Recognizes the Sequence RHRK20VLRD within the N-terminus of Histone H4 and Mono-methylates Lysine 20,  J. Biol  Chem  280, 30025-31 (times cited 16)

58.  Yinliang Yin2 and Guang Zhu* (2006) SET8 recognizes the sequence RHRKB20BVLRDN within the N-terminus of histone H4 and mono-methylates lysine 20. Neurosignals 15, 52

59.  Denise S.B. Chan, Lai-On Chu, Ka-Ming Lee, Priscilla H.M. Too, Kit-Wan Ma, Kong-Hung Sze5, Guang Zhu, Pang-Chui Shaw1 and Kam-Bo Wong12007Interaction between trichosanthin, a ribosome-inactivating protein, and the ribosomal stalk protein P2 by chemical shift perturbation and mutagenesis analyses. Nucleic Acids Research, 35, 1660-72 (times cited 20)

60.  Xuejun Yao3Guang Zhu*2008Sensitivity Improvement of 2D HOESY by Presaturation, Chinese Journal of Magnetic resonance. 25,4, 24-26 (cited 3)

61.  Bo Zhou3, Lihong Chen3, Xing Wu3, Jing Wang3, Yinliang Yin2 and Guang Zhu* (2008) MH1 Domain of SMAD4 Binds N-terminal Residues of the Homeodomain of Hoxc9, BBA-Proteins&Proteomics 1784(5):747-52 (times cited 7)

62.  Yinliang Yin2, Vivian C. Yu, Guang Zhu* and Donald C. Chang1* (2008) SET8 plays a role in controlling G1/S transition by blocking lysine acetylation in histone through binding to H4 N-terminal tail.  Cell Cycle  7 (10)  1423-1432   (times cited 18)

63.  Guang Zhu* and Xuejun Yao (2008) TROSY-based NMR experiments for large biomolecules, Prog. in NMR Spectroscopy. 52 49-68 (times cited 3)

64.  Shaw, Pang-Chui1; Ma, Meiji; Mak, Amanda; Wong, Yuen-Ting; Tung, Christine; Guang Zhu, Au, Shannon; Wong, Kam-Bo1 (2009) The C-terminal fragment of the ribosomal P protein complexed to trichosanthin reveals the interaction between the ribosome-inactivating protein and the ribosome, Nucleic Acids Research, Vol. 37, No. 2 602-610.  (times cited 13)

65.  Lee, Ka-Ming; Yu, Conny Wing-Heng; Chan, Denise So-Bik; Chiu, Teddy Yu-Hin; Zhu, Guang; Sze, Kong-Hung; Shaw, Pang-Chui; Wong, Kam-Bo1 (2010) Solution Structure of the Dimerization Domain of Ribosomal Protein P2 Provides Insights for the Structural Organisation of Eukaryotic Stalk, Nucleic Acids Research, 38 (15), 5206-5216 (times cited 6)

66.  Zhun Wei3, Changdong Liu3, Bo Zhou2, Naining Xu3, Chun Liang1* and Guang Zhu* (2010) Characterization and structure determination of the Cdt1 binding domain of human minichromosome maintenance (Mcm) 6. J. Biol. Chem. 285 (17) 12469-73.(times cited 8)

67.  Changdong Liu3, Zhun Wei3, Guang Zhu* (2010) 1H, 15N and 13C chemical shift assignments of the Cdt1 binding domain of human Mcm6, Biomolecular NMR Assignments (BNMR), 4, 231-233

68.  Zhangxi Hao4, Min Tan, Changdong Liu3, Feng Rui5 Endo Wang1* and Guang Zhu* (2010) Studying base pair open-close kinetics of tRNALeu by TROSY-based proton exchange NMR spectroscopy, FEBS Letter 584,4449-52.

69.  Zhangxi Hao4, Changdong Liu3, Feng Rui5, Endo Wang1 and Guang Zhu* (2010) 1H, 15N chemical shift assignments of the imino groups in the base pairs of Escherichia coli tRNALeu (CAG), Biomolecular NMR Assignments (BNMR), 5 (1), pp. 71-74

70.  Jingjing Zhang, Lan Yu, Xing Wu, Lan Zou, K.L.Sou, Zhun We4, Xue Cheng, Guang Zhu*, Chun Liang* (2010) The interacting domains of hCdt1 and hMcm6 involved in the chaomating loading of the Mcm comple in human cells. Cell cycle 9, 24, 7-6.

71.   Lihong Chen,Changdong Liu3, Feng Rui, Guang Zhu* (2011) 1H, 15N and 13C chemical shift assignments of the SH2 domain of human Tensin2, Biomolecular NMR Assignments (BNMR) 5(2):211-4

72.  Guang Zhu* (2012) invited book editor, NMR of Proteins or Small Biomolecules, Topics in Current Chemistry, Volume 326, Springer.

73.  Kong Hong Sze, Qinglin Wu, Ho sum Tse, Guang Zhu*(2012) Dynamic Nuclear Polarization: New Methodology and Applications. Chapter 7, Springer. (Top Curr Chem ) 326:215-242

74.  Changdong Liu2, Rentian Wu, Bo Zhou, Jiafeng Wang, Bik Tye1, Chun Liang*1 and Guang Zhu* (2012) Structural Insights into the Cdt1-Mediated Mcm2-7 Chromatin Loading. Nucleic Acids Res. 40, 3208-17.

75.  Bo Zhou, Changdong Liu, Zhiwen Xu, and Guang Zhu* (2012) Structural basis for homeodomain recognition by the cell cycle regulator Geminin, Proc. Natl. Acad. Sci. U S A. 109 23 8931-8936

76.  Lihong Chen3, Changdong Liu3, Frankie Chi Fat Ko1, Naining Xu3, Irene Oi-lin Ng3, Judy Wai Ping Yam3* and Guang Zhu* (2012) Solution Structure of the Phosphotyrosine Binding (PTB) Domain of Human Tensin2 Protein in Complex with Deleted in Liver Cancer 1 (DLC1) Peptide Reveals a Novel Peptide Binding Mode, J Biol. Chem. 287, 26104–26114

77.  Yueru SunShuhui YinYitao FengJie LiJiahai ZhouChangdong Liu2, Guang Zhu and  Zhihong Guo (2014Molecular Basis of the General Base Catalysis of an α/β-Hydrolase Catalytic Triad, Journal of Biological Chemistry ( in press)

78.  Bo Zhou, Changdong Liu and Guang ZHU* (2014) 1H, 15N and 13C chemical shift assignments of the homeodomain of Hoxc9 in complex with cell cycle regulator Geminin, Biomolecular NMR Assignments (BNMR),(submitted)

79.  Rui Feng, Changdong Liu, Bo Zhou, Yuning Hong, Benzhong Tang and Guang Zhu* (2014) The Chair-type Structure of the Intramolecular Human Telomeric G-quadruplex, Nucleic Acids Res. (in revision)

80.  Naining Xu, Changdong Liu, Capel Tunglun Lee, Bo Zhou, Yanyan Geng and Guang ZHU* (2014) Structural analysis of human Orc6-DNA complex, (in preparation)


1 Collaborator, 2 My Postdoc, 3 My PG Student, 4 My Research Assistant, 5 My Technician, * Corresponding Author.


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