Division of Life Science
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Yusong GUO

PhD Carnegie Mellon University
Assistant Professor

Email: guoyusong@ust.hk

Division of Life Science, HKUST

Dr. Guo's Laboratory page

Advertisment for Research Assistant or Postdoctoral Research Associate

Research Interests

Molecular mechanism that regulates sorting of planar cell polarity signaling receptors at the trans Golgi network

Many epithelial tissues not only exhibit an apical-basolateral polarity but also acquire a second axis of polarity perpendicular to the apical-basal axis, referred to as planar cell polarity (PCP). Defects in PCP signaling are associated with a variety of human diseases including cancer metastasis and congenital heart disease. The establishment of PCP is controlled by a group of conserved signaling receptors that are localized asymmetrically on opposing cellular boundaries. This asymmetric localization is critical for accurate propagation of the PCP signaling process. We are interested in understanding how PCP signaling receptors are delivered to the plasma membrane as well as how their asymmetric localizations are established.

The trans Golgi network (TGN) is an essential transport hub in the biosynthetic transport pathway. At the TGN, elaborate cargo sorting machineries function to sort newly-synthesized proteins into distinct transport vesicles that are delivered to various downstream destinations. TGN sorting is an important step for accurate targeting of various proteins to their specific localizations. We aim to identify the molecular machineries that mediate sorting of the asymmetrically localized PCP signaling receptors into vesicles at the TGN utilizing cutting edge technologies in biochemistry, cell biology and fluorescent imaging. We also aim to analyze whether protein sorting at the TGN is important for establishing the asymmetric localizations of PCP signaling receptors as well as for the propagation of the PCP signaling process.

Representative Publications

  1. Ma T, Li B, Wang R, Lau PK, Huang Y, Jiang L, Schekman R, Guo Y*. A mechanism for differential sorting of planar cell polarity proteins Frizzled6 and Vangl2 at the trans-Golgi Network. Journal of Biological Chemistry. 2018 Apr 17.
  2. Guo Y*, Yang F, Tang X. An Overview of Protein Secretion in Yeast and Animal Cells. Methods Mol Biol. 2017;1662:1-17.
  3. Ma Y, Feng L, Guo Y, Gong H. Statistical analysis and probabilistic verification of stress-induced signalling pathways. International Journal of Data Mining and Bioinformatics. 2016 Feb; 14(2): 120-138.
  4. Guo Y, Sirkis DW, Schekman R. Protein Sorting at the trans-Golgi Network. Annu Rev Cell Dev Biol. 2014 Aug 18.
  5. Guo Y, Zanetti G, Schekman R. A novel GTP-binding protein-adaptor protein complex responsible for export of Vangl2 from the trans Golgi network. eLife. 2013; 2:e00160. (highlighted in Nature Cell Biology and in the Annual Review of Cell and Developmental Biology)
  6. Guo Y and Linstedt AD. Binding of the vesicle docking protein p115 to the GTPase Rab1b regulates membrane recruitment of the COPI vesicle coat. Cellular Logistics 2013 Dec; 3:1, e27609.
  7. Gong H, Guo Y, Linstedt AD and Schwartz R. Discrete, continuous and stochastic models of protein sorting in the Golgi. Phys Rev E Stat Nonlin Soft Matter Phys. 2010 Jan; 81(1 Pt 1):011914.
  8. Srinivasa G, Fickus MC, Guo Y, Linstedt AD and Kovačević J. Active mask segmentation of fluorescence images. IEEE Transactions on Image Processing. 2009 Aug; 18(8):1817-1829.
  9. Guo Y, Punj V, Sengupta D and Linstedt AD. Coat-tether interaction in Golgi organization. Molecular Biology of the Cell. 2008 July; 19(7):2830-43. (highlighted in Incytes from MBC)
  10. Srinivasa G, Fickus M, Gonzales-Rivero MN, Hsieh SY, Guo Y, Linstedt AD and Kovačević J. Active mask segmentation for the cell-volume computation and Golgi-body segmentation of HeLa cell images. Proc. IEEE Intl. Symp. Biomed. Imaging, Paris, France, 2008 May; pp. 348-351.
  11. Guo Y and Linstedt AD. COPII-Golgi protein interactions regulate COPII coat assembly and Golgi size. Journal of Cell Biology. 2006 July; 174(1): 53-63. (highlighted in Faculty of 1000 and in the Annual Review of Cell and Developmental Biology)
  12. Guo Y, Kong L, Wang Y, Huang Z. Antidepressant evaluation of polysaccharides from a Chinese herbal medicine Banxia-houpu decoction. Phytotherapy Research. 2004 March;18(3): 204-207.
  13. Kong L, Yang C, Ge F, Wang H, Guo Y. A Chinese herbal medicine Ermiao wan reduces serum uric acid level and inhibits liver xanthine dehydrogenase and xanthine oxidase in mice. Journal of Ethnopharmacology. 2004 Aug; 93(2-3): 325-330.
  14. Wang H, Ge F, Guo Y, Kong L. Effects of aqueous extract in herba of Lysimachiachristinae on hyperuricemia in mice. ZhongguoZhong Yao ZaZhi. 2002; 27(12): 939-41, 944.