Division of Life Science
 
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Bik-Kwoon YEUNG TYE

PhD M.I.T.
Part-time Visiting Professor (Professor Emeritus Cornell University)

Email: biktye@ust.hk

 

Research Interests

The Eukaryotic DNA Replication Structure and Dynamics Group focuses on the study of the structures and functions of the eukaryotic DNA replication machinery. We purify endogenous macromolecular DNA replication complexes at different stages of assembly from yeast cells and build atomic models of their structures based on high resolution CryoEM. This project is carried out in collaboration with the CryoEM group led by Professor Ning Gao at Tsinghua University. We also study the subcellular organization of the replication machineries within the nucleus during different stages of the cell division cycle using superresolution microscopy (STORM) and SIM lattice light sheet. This project is made possible by microscopes custom-built by our local experts, Professor Shengwang Du and Mr. Zhou Teng in the Physics Department.

 

 

Representative Publications

http://www.ncbi.nlm.nih.gov/pubmed?term=Tye BK

  1. Zhai, YL#, Li, N., Jiang, H., Huang, X., Gao, N. #, Tye, BK# (2017) Unique Roles Played by each of the Non-Identical MCM Subunits in DNA Replication Licensing. Mol Cell 67: 168-179.

  2. Zhai, YL, Chen, E, Wu, H., Li, N., Yung, P.; Gao, N.#, Tye, BK# (2017) Open-Ringed Structure of the Cdt1-Mcm2-7 Complex as a Precursor of the MCM Double Hexamer. Nat Struct & Mol Biol. 24: 300-308

  3. Li, N., Zhai, YL, Zhang Y., Li, W., Yang, M., Lei, J., Tye, BK#, Gao, N#. (2015) Structure of the Eukaryotic Minichromosome Maintenance Complex at 3.8Å. Nature 524:186-91. #co-corresponding authors

  4. Li, X.C. and Tye, B.K. (2011) Ploidy Dictates Repair Pathway Choice under DNA Replication Stress. Genetics. 187: 1031-1040.

  5. Liachko, I., Bhaskar, A., Lee, C., Chung, S.C., Tye, B.K.*, and Keich, U. (2010) A Comprehensive Genome-Wide Map of Autonomously Replicating Sequences in a Naive Genome. PLoS Genet. 6:e10000946.

  6. Lee, C., Liachko, I., Bouten, R., Kelman, Z., and Tye, B.K. (2010) Alternative Mechanisms for Coordinating Polymerase alpha and MCM Helicase. Mol Cell Biol. 30(2):423-35.

  7. Li, X. C., Schimenti, J.C. and Tye, B.K. (2009) Aneuploidy and Improved Growth are Coincident but not Causal in a Yeast Cancer Model. PLoS Biol 7:e1000161.

  8. Shima, N., Alcaraz, A., Liachko, I., Buske, T. R., Andrews, C. A., Munroe, R. J., Hartford, S. A., Tye, B.K., Schimenti, J. C. (2007) A viable allele of Mcm4 causes chromosome instability and mammary adenocarcinomas in mice. Nat Genet 39: 93-98.

  9. Maine, G., Sinha, P. and Tye, B.K. (1984) Mutants of S. cerevisiae defective in the maintenance of minichromosomes. Genetics 106: 365–385.

 

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