Division of Life Science
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Zilong WEN

PhD Rockefeller

Email: zilong@ust.hk

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Research Interests

Cellular and molecular basis of hematopoietic stem cell and tissue-resident macrophage development; the roles of microglia and Langerhans cells in organ development and tissue regeneration.

Wen’s laboratory is keen to understand the developmental mechanism that establishes the cellular components of the blood system and how their dysregulation incurs diseases by utilizing zebrafish (Danio rerio), a tiny tropical fish whose blood system shares many similarities to human. A combination of photo-inducible lineage tracing, time-lapse imaging and devising genetic strategies will be employed to investigate the origin, migration and colonization, fate determination and function of hematopoietic stem cells, microglia and Langerhans cells in the intact developing and adult zebrafish with highest possible cellular resolution. The ultimately goal is to uncover novel cellular and molecular principles that organize the formation, maintenance and function of these hematopoietic cells under physiological and pathological states.


Representative Publications

  1. Tian Y, Xu J, Feng SC, He SC, Zhao SZ, Zhu L, Jin W, Dai YM, Luo LF, Qu JY, and Wen ZL. (2017) The first wave of T lymphopoiesis in zebrafish arises from aorta endothelium independent of hematopoietic stem cells. The Journal of Experimental Medicine 214:3347-3360.

  2. Yu T, Guo WL, Tian Y, Xu J, Chen JH, Li L, and Wen ZL. (2017) Distinct regulatory networks control the development of macrophages of different origins in zebrafish. Blood 129:509-519.

  3. Dai YM, Zhu L, Huang ZB, Zhou MY, Jin W, Liu W, Xu MC, Yu T, Zhang YY, Wen ZL*, Liao WJ*, and Zhang WQ*. (2016) Cebpα is essential for the embryonic myeloid progenitor and neutrophil maintenance in zebrafish. Journal of Genetics and Genomics 43:593-600 (*Co-corresponding author).

  4. Xu J, Wang TN, Wu Y, Jin W, and Wen ZL. (2016) Microglia colonization of developing zebrafish midbrain is promoted by apoptotic neuron and lysophosphatidylcholine. Developmental Cell 38:214-222.

  5. Jin H, Huang ZB, Chi YL, Wu M, Zhao LF, Zhou RY, Xu J, Zhen FH, Lan YH, Li L, Zhang WQ, Wen ZL*, and Zhang YY*. (2016) cMyb acts in parallel and cooperatively with Cebp1 to regulate neutrophil maturation in zebrafish. Blood 128:415-426 (Co-corresponding author)

  6. Wei YL, Xu J, Zhang WQ, Wen ZL*, and Liu F*. (2016) RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish. Development 143:2103-2110 (*Co-corresponding author)

  7. Xu J, Zhu L, He SC, Wu Y, Jin W, Yu T, Qu JY, and Wen ZL. (2015) Temporal-spatial resolution fate mapping reveals distinct origins for embryonic and adult microglia in zebrafish. Developmental Cell 34:632–641.

  8. Yan B, Han PD, Pan LF, Lu W, Xiong JW, Zhang MJ, Zhang WQ, Li L, and Wen ZL. (2014) Il-1β and reactive oxygen species differentially regulate neutrophil directional migration and basal random motility in a zebrafish injury-induced inflammation model. The Journal of Immunology 192:5998-6008.

  9. Zhen FH, Lan YH, Yan B, Zhang WQ, and Wen ZL. (2013) Hemogenic endothelium specification and hematopoietic stem cell maintenance employ distinct Scl isoforms. Development 140:3977-3985. (F1000 recommended)

  10. Sun JP, Liu W, Li L, Chen JH, Wu M, Zhang YY, Leung AYH, Zhang WQ*, Wen ZL*, and Liao WJ*. (2013) Suppression of Pu.1 function results in expanded myelopoiesis in zebrafish. Leukemia 27:1913-1917. (*Co-corresponding author)

  11. Li XL, Lan YH, Xu J, Zhang WQ, and Wen ZL. (2012) SUMO1-activating enzyme subunit 1 is essential for the survival of hematopoietic stem/progenitor cells in zebrafish. Development 139:4321-4329.

  12. Xu J, Du L, and Wen ZL. (2012) Myelopoiesis during zebrafish early development. Journal of Genetics and Genomics 39:435-442. (Invited review)

  13. Li L, Yan B, Shi YQ, Zhang WQ, and Wen ZL. (2012) Live imaging reveals differing roles of macrophages and neutrophils during zebrafish tail fin regeneration. The Journal of Biological Chemistry 287:25353-25360.

  14. Jin H, Li L, Xu J, Zhen FH, Zhu L, Liu PP, Zhang MJ, Zhang WQ, and Wen ZL. (2012) Runx1 regulates embryonic myeloid fate choice in zebrafish through a negative feedback loop inhibiting Pu. 1 expression. Blood 119:5239-5249.

  15. Zhang YY, Jin H, Li L, Qin X-F, and Wen ZL. (2011) cMyb regulates hematopoietic stem/progenitor cell migration during zebrafish hematopoiesis. Blood 118:4093-4101.

  16. Du LS, Xu J, Li XL, Ma N, Liu YM, Peng JR, Osato M, Zhang WQ, and Wen ZL. (2011) Rumba and Haus3 are essential factors for maintenance of hematopoietic stem/progenitor cells during zebrafish hematopoiesis. Development 138:619-629.

  17. Li L, Jin H, Xu J, Shi YQ, and Wen ZL. (2011) Irf8 regulates macrophage versus neutrophil fate during zebrafish primitive myelopoiesis. Blood 117:1359-1369.

  18. Jin H, Sood R, Xu J, Zhen FH, Liu PP, and Wen ZL. (2009) Definitive hematopoietic stem cells manifest distinct differentiation output in the zebrafish VDA and PBI. Development 136:647-654.

  19. Huang HH, Ruan H, Aw YM, Hussain A, Guo L, Gao C, Qian F, Leung T, Song H, Kimelman D, Wen ZL*, and Peng JR*. (2008) Mypt1-mediated spatial positioning of Bmp2-producing cells is essential for liver organogenesis. Development 135:3209-3218. (*Co-corresponding author)

  20. Liu YM, Du LS, Osato M, Teo EH, Qian F, Jin H, Zhen FH, Xu J, Guo L, Huang HH, Chen J, Geisler R, Jiang YJ, Peng JR, and Wen ZL. (2007) The zebrafish udu gene encodes a novel nuclear factor and is essential for primitive erythroid cell development. Blood 110:99-106.

  21. Jin H, Xu J, and Wen ZL. (2007) Migratory path of definitive hematopoietic stem/progenitor cells during zebrafish development. Blood 109:5209-5214.

  22. Qian F, Zhen FH, Xu J, Huang M, Li WY, and Wen ZL. (2007) Distinct functions for different scl isoforms in zebrafish primitive and definitive hematopoiesis. PLoS Biology 5:1110-1119.

  23. Jin H, Xu J, Qian F, Du LS, Tan CY, Lin ZX, Peng JR, and Wen ZL. (2006) The 5’ zebrafish scl promoter targets transcription to the brain, spinal cord, hematopoietic and endothelial progenitors. Developmental Dynamics 235:60-67.

  24. Qian F, Zhen FH, Ong CT, Jin S-W, Soo HM, Stainier DYR, Lin S, Peng JR and Wen ZL. (2005) Microarray analysis of zebrafish cloche mutant using amplified cDNA and the identification of potential hematopoietic-endothelial target genes. Developmental Dynamics 233:1163-1172.

  25. Lo J, Lee S, Xu M, Liu F, Ruan H, Eun A, He Y, Wang W, Wen ZL*, and Peng JR*. (2003) 15,000 unique zebrafish EST clusters and their use in microarray for profiling gene expression patterns during embryogenesis. Genome Research 13:455-466. (*Co-corresponding author)

  26. Huang M, Qian F, Hu Y, Ang C, Li Z, and Wen ZL. (2002) The chromatin-remodelling factor BRG1 selectively activates a subset of interferon α-inducible genes. Nature Cell Biology 4:774-781.

  27. Liu F and Wen ZL. (2002) Cloning and expression pattern of the lysozyme C gene in zebrafish. Mechanisms of Development 113:69-72.

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