Prof. Wong’s major interests are currently focused on the delineation of the mechanisms of cell signaling, particularly those involving the G protein-coupled receptors (GPCRs). The functional capabilities of GPCRs that bind opioid peptides, melatonin, or chemokines, and their G protein-coupling specificities are examined in different cellular and animal systems. Delineation of interacting domains on the GPCRs and their associated G proteins provides a framework for understanding the fidelity of cell signaling and the creation of novel tools for drug discovery. Signal integration at the level of effectors such as adenylyl cyclase, phospholipase C, and mitogen-activated protein kinases are examined in cultured mammalian cells. In addition, the roles of GPCRs, G proteins, and RGS proteins in the regulation of cell proliferation and differentiation are examined using a variety of immunological, biochemical, pharmacological, and molecular biology techniques.
Li Y, Song J, Chung SK, and Wong YH (2017) RGS19 upregulates Nm23-H1/2 metastasis suppressors by transcriptional activation via the cAMP/PKA/CREB pathway. Oncotarget8: 69945-69960.
Chan ASL, Lau WWI, Szeto A, Wang J, and Wong YH (2016) Differential regulation of CXCL8 production by different G protein subunits with synergistic stimulation by Gi- and Gq-regulated pathways. J. Mol. Biol. 428: 3869-3884.
Hu Y, Chan KH, He X, Ho MKC, New DC, and Wong YH (2014) Synthesis and functional characterization of substituted isoquinolinones as MT2-selective melatoninergic ligands. PLoS ONE 9: e113638.
Wang Y, Tong Y, Tso PH, and Wong YH (2013) Regulator of G protein signaling 19 suppresses Ras-induced neoplastic transformation and tumorigenesis. Cancer Lett. 339: 33-41.
Lee MMK, Chui RKS, Tam YS, Lau HYA, and Wong YH (2012) CCR1-mediated STAT3 tyrosine phosphorylation and CXCL8 expression in THP-1 macrophages involves pertussis toxin-insensitive G14/16 signaling and interleukin-6 release. J. Immunol. 189: 5266-5276.